LPA Laboratory Equipment: PCR Hood

Polymerase Chain Reaction (PCR) hood is a workstation enclosed by three sides.

Use of Polymerase Chain Reaction (PCR) Hood:

• Used in the Master Mix Reagent Preparation and DNA Amplification Room
• Ultraviolet (UV) light (Figure) in PCR hood denatures nucleic acids​
• Used to decontaminate reagents and equipment prior to PCR reaction​
• Provides protection against exogenous DNA, contaminants or inhibitors from entering the reaction tubes

General Instructions on Maintenance

• Always switch off the appliance before any maintenance activity​.
• Always record every maintenance activity performed​.

Maintenance ​​

• Clean outer and inner surfaces with freshly prepared 1% sodium hypochlorite​.
• Replace UV bulbs and filters according to manufacturer recommendations​.

Resources

• Molecular Detection of Drug-Resistant Tuberculosis by Line Probe Assay.

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