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LPA Troubleshooting for Other Possible Reasons
Learning Objectives-
Issue #1:
Absence of Conjugate Control (CC) and Amplification Control (AC) in a negative control (Figure 1).
Figure 1: Absence of CC and AC in a Negative Control
Table 1 below lists the reasons and troubleshooting for the absence of CC and AC in a negative control.
REASONS |
TROUBLESHOOTING |
---|---|
Mistakes during setup |
The test is invalid – Repeat test |
Performance of amplification reaction |
|
Presence of amplification inhibitors |
Issue 2:
Absence of locus control for a specific drug, with presence of CC and M. tuberculosis complex (TUB) band (Figure 2).
Figure 2: Absence of locus control for a specific drug, with presence of CC and TUB band
Table 2 below lists the reasons and troubleshooting for the absence of locus control for a specific drug, with the presence of CC and TUB band.
REASONS |
TROUBLESHOOTING |
---|---|
Mutation or deletions in locus control region |
|
Complete or partial deletion of a target gene |
Resources
- GenoType MTBDR plus ver 2.0 kit, Instructions for Use.
- GenoType MTBDRsl VER 2.0 kit, Instructions for Use.
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