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Issue #1:

Absence of Conjugate Control (CC) and Amplification Control (AC) in a negative control (Figure 1).

 

Figure 1: Absence of CC and AC in a Negative Control

 

 

Table 1 below lists the reasons and troubleshooting for the absence of CC and AC in a negative control​.

 

Table 1: Reasons and troubleshooting for the absence of CC and AC in a Negative Control​ 

REASONS

TROUBLESHOOTING

Mistakes during setup​

The test is invalid – Repeat test​

Performance of amplification reaction​

Presence of amplification inhibitors​

 

Issue 2: 

Absence of locus control for a specific drug, with presence of CC and M. tuberculosis complex (TUB) band (Figure 2).

Figure 2: Absence of locus control for a specific drug, with presence of CC and TUB band

 

 

Table 2 below lists the reasons and troubleshooting for the absence of locus control for a specific drug, with the presence of CC and TUB band​.

 

Table 2: Reasons and troubleshooting for the absence of locus control for a specific drug, with the presence of CC and TUB band​

REASONS 

TROUBLESHOOTING 

Mutation or deletions in locus control region​

  • The test is indeterminant - Repeat test​
  • Sequencing may be requested to identify the specific mutation​

Complete or partial deletion of a target gene

 

 

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