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Use the sterile transfer pipette provided in the CBNAAT kit to draw liquefied sample into the transfer pipette

  • The minimum amount to be loaded into the cartridge is 2 ml
  • Do not process the sample if there is insufficient volume
  • Open the cartridge lid
  • Transfer the sample into the open port (Figure 1) of the CBNAAT cartridge and dispense slowly to minimize the risk of aerosol formation

 

Figure 1: CBNAAT cartridge (top view) to show the open port
(SOP for GeneXpert MTB/RIF)

  • Discard the transfer pipette
  • Snap the lid shut to close firmly
  • Turn on the CBNAAT instrument
  • Open the instrument module door, which displays the blinking green light
  • Load the cartridge and close the door of the module firmly
  • The test should be started within 30 minutes of adding liquefied sample to the cartridge
  • The remaining liquefied sample may be kept for up to 12 hours at 2-8°C (for repeat testing)

 

Video file

Video 1: CBNAAT loading

 

 

Video file

Video 2: Sample Loading (Demonstration)

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