Preparation of PANTA for MGIT TB Cultures

Preparation of PANTA is the first step when inoculating and incubating MGIT 960 tubes for TB cultures. The preparation steps are shown below (Figure 1).

Figure 1: Steps for Preparation of PANTA

Figure 2: Reconstitution of PANTA with growth supplement using a micropipette

NOTE:

1. Reconstituted PANTA can be used for up to 5 days if stored at 2 to 8^ºC, but do not freeze.
2. Each PANTA vial is used for 15-18 MGIT tubes (BACTEC MGIT 960).

Please click the video below to see the full procedure for inoculating and incubating MGIT tubes for TB cultures:

https://empowerschoolofhealth-my.sharepoint.com/:v:/g/personal/admin_empowerschoolofhealth_org/EXh5-goFTP5PvXwEDDp-r4UBvpE3fW4G4OmBRzP296LfTg?e=keEf8t

Resources

• MGIT Procedure Manual, Mycobacteria Growth Indicator Tube (MGIT) Culture and Drug Susceptibility Demonstration Projects, FIND Training Manual.

Kindly provide your valuable feedback on the page to the link provided HERE