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  • Storing a sputum sample

    Learning Objectives

    The learner will be able to describe the storage conditions for a sputum sample for microscopy, molecular tests and liquid culture.

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Storage conditions of sputum sample can effect the test results.

  • Sputum samples should be transported to the laboratory as soon as possible after collection.
  • It is the responsibility of Laboratory Technician (LT) and Senior TB Laboratory Supervisor (STLS) to ensure proper storage and transport of sputum specimens.
  • Sputum is stored to preserve the specimen quality.
  • The stored sputum samples should not be frozen.

Storage of Sputum Samples

For microscopy

  • For smear microscopy, sputum specimens should be examined on the same day and not later than 2 days after collection.
  • If delay is unavoidable, the sputum collected should be stored in a cool place/ refrigerated at 4°C to inhibit the growth of unwanted microorganisms.
  • Stored sputum samples should be protected from light and heat to prevent liquefaction of the sample, else it makes the selection of mucopurulent part of the sample difficult.
  • Samples received over holidays/weekends should be stored in a cool place/ refrigerated at 4°C.

For liquid culture

  • Sputum should be stored in a cool place/ refrigerated at 4°C to inhibit the growth of unwanted microorganisms; not later than 3 days after collection.

For molecular tests - Nucleic Acid Amplification Test (NAAT) and Line Probe Assay (LPA)

  • Sputum must be stored by refrigerating at 4°C to inhibit the growth of unwanted microorganisms and transported in cool chain to the nearest molecular laboratory. It should not be stored beyond one week at 4°C.

Resources

Technical and Operational Guidelines; Chapter 3: Case finding and diagnosis strategy

PMDT Guidelines 2021

Assessment

Question Answer 1 Answer 2 Answer 3 Answer 4 Correct answer Correct explanation Page id Part of Pre-test Part of Post-test
The sputum collected expecting a time delay for processing should be stored in cool place/ refrigerated at 4°C. True False     1

The sputum collected expecting a time delay for processing should be stored in cool place/ refrigerated at 4°C.

 

 

  Yes Yes

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