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ZN Microscopy: Staining Process
Learning ObjectivesDescribe the Staining Process for ZN Microscopy:
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After air drying and heat-fixing of the smear, it needs to be stained for the identification of MTB during microscopy.
The reagents required for the ZN staining procedure are shown in Figure 1. The steps involved in the staining procedure are shown in Figure 2.
Figure 1: Reagents Required for ZN Staining
Figure 2: Steps involved in ZN staining procedure
For a visual representation of the above-mentioned steps, please click the video below.
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Laboratory Diagnosis by Sputum Smear Microscopy, GLI Initiative
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Question | Answer 1 | Answer 2 | Answer 3 | Answer 4 | Correct answer | Correct explanation | Page id | Part of Pre-test | Part of Post-test |
When staining ZN slides, it is important to heat the slides until it begins to boil. | True | False | 2 | When staining ZN slides, it is important to heat the slides gently until you see vapours, but do not boil. | Yes | Yes |
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