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Linked Node
Quality Control of the MGIT DST Inoculum
Learning Objectivesdilution, suspension, inoculation, positive/negative controls
Quality control of MGIT DST inoculum is essential for quality DST results. The different aspects of inoculum preparation include:
1. Using the right inoculum
2. Using pure cultures only
3. Using fresh cultures
4. Using slant < 14 days
Quality aspects for Positive MGIT tube include:
1. Time period should be plus 1 day to 5 days from the date of positivity
2. Homogeneous inoculum
3. Using sterile glass beads and vortex to break up organism clumps
4. Too high or too low an inoculum may give error results or un-interpretable results
5. Preparing dilutions according to the procedure
6. Using an accurate pipette to add inoculum to tubes
Resource
GLI Practical Guide to TB Laboratory Strengthening
Assessment
Question
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Answer 1
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Answer 2
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Answer 3
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Answer 4
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Correct answer
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Correct explanation
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Page id
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Part of Pre-test
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Part of Post-test
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Quality control of MGIT DST inoculum includes which of the following?
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High inoculum
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Time period to be plus 1 day to 5 days from the date of positivity
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Non-homogenous suspension
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21-day-old culture
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2
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The time period should be plus 1 day to 5 days from the date of positivity.
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YES
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YES
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