EQA for Fluorescent Microscopy [FM]

The External Quality Assessment (EQA) for Fluorescent Microscopy (FM) follows the same basic principles as for sputum smear microscopy using Ziehl-Neelsen (ZN) staining:

1. On-site Evaluation (OSE)
2. Panel Testing
3. Random Blinded Re-checking

Differences between EQA for FM and sputum smear stained by ZN method:

• The stained slides for FM are stored in the dark as fluorescence is prone to fading over time.
• The slides for FM are viewed under 40X without use of immersion oil.
• Acid-fast bacilli appear bright yellow against the dark background in FM.

During the OSE visit, the Senior Laboratory Supervisor (STLS) will cross check all positive smears and selected negative smears.

Resources

• Manual for Sputum Smear Fluorescence Microscopy.
• Module for Laboratory Technicians.

Kindly provide your valuable feedback on the page to the link provided HERE