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SIRE Susceptibility Testing
Learning ObjectivesDescribe components of SIRE kit
The BACTEC MGIT 960 susceptibility testing for Streptomycin (S), Isoniazid (I), Rifampin (R) and Ethambutol (E), called SIRE, is a rapid and qualitative method that uses critical test concentrations of the four drugs.
Components of SIRE Kit
The kit has one vial for each of the drugs (S, I, R and E) and 8 vials of the MGIT 960 SIRE Supplement.
Drugs:
|
Drug |
Approximate lyophilized drug per vial
|
|
Streptomycin (S) |
332 µg |
|
Isoniazid (I) |
33.2 µg |
|
Rifampin (R) |
332µg |
|
Ethambutol (E) |
1660µg |
SIRE Supplement: It differs from the MGIT Growth Supplement and contains the following:
|
Bovine Albumin |
50g/l
|
|
Dextrose |
20g/l
|
|
Catalase |
0.03g/l
|
|
Oleic Acid |
0.6g/l
|
Resource
Mycobacteriology Laboratory Manual
Assessment
|
Question |
Answer 1 |
Answer 2 |
Answer 3 |
Answer 4 |
Correct answer |
Correct explanation |
Page id |
Part of Pre-test |
Part of Post-te |
| Which of the following is NOT a component of the SIRE kit? | Streptomycin (S) | Isoniazid (I) | Rifabutin (R) | Ethambutol (E) | Answer 3 | Components of the SIRE Kit are Streptomycin (S), Isoniazid (I), Rifampin (R), and Ethambutol (E). |
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