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Principle of procedure

  • A centrifuge separates particles in a liquid by sedimentation. Dense particles sediment first, followed by lighter particles
  • To obtain a high sedimentation efficiency of 95%, it is necessary to centrifuge the TB specimens at 3,000 g for 15-20 minutes
  • The high speed and time used to achieve effective sedimentation efficiency lead to heat build-up in the centrifuge and the specimens.  To prevent cell death due to overheating, the use of a refrigerated centrifuge is recommended

 

How to use refrigerated centrifuges?

Select type of tube, based on:

  • G force used for swing-out bucket
  • Time of centrifugation 
  • Temperature
  • Compatibility with specimen 
  • Chemically inert

 

Visually inspect tubes  

Do not use if tubes have:

  • Cracks
  • Scratches
  • Chipped rims

 

Fill tubes only to the recommended volume

  • Do not overfill tubes
  • High g forces can drive liquid up to the cap and cause overflowing

 

Before using centrifuge

  • Inspect the interior of the bowl for stains
  • Inspect rotors and buckets for cracks and signs of corrosion
  • Always check the silicon O ring for any wear & tear of the aerosol lid
  • Check log book to ensure centrifuge is in good working condition
  • Pre-cool centrifuge prior to use

 

Use of safety centrifuge

  • Centrifuge load MUST always be balanced to avoid the risk of damage
  • Add a balance tube with the same volume of water if processing an odd number of specimens
  • Select the required time and g force or RCF 
  • Stop centrifuge immediately if any abnormal noise and/or shaking is noticed
  • Never use the brake to stop a normal operating centrifuge for TB specimen processing
  • ALWAYS open the sealed buckets inside the biosafety cabinet (BSC)

 

Microcentrifuge

  • Always balance the rotor 
  • Use high-quality screw-capped tubes, which will not leak or break during centrifugation 
  • When using a microcentrifuge for DNA extraction, the microcentrifuge should be loaded and unloaded inside the BSC
  • Remove and replace the rotor carefully

 

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