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How to Use Refrigerated Centrifuges
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Principle of procedure
- A centrifuge separates particles in a liquid by sedimentation. Dense particles sediment first, followed by lighter particles
- To obtain a high sedimentation efficiency of 95%, it is necessary to centrifuge the TB specimens at 3,000 g for 15-20 minutes
- The high speed and time used to achieve effective sedimentation efficiency lead to heat build-up in the centrifuge and the specimens. To prevent cell death due to overheating, the use of a refrigerated centrifuge is recommended
How to use refrigerated centrifuges?
Select type of tube, based on:
- G force used for swing-out bucket
- Time of centrifugation
- Temperature
- Compatibility with specimen
- Chemically inert
Visually inspect tubes
Do not use if tubes have:
- Cracks
- Scratches
- Chipped rims
Fill tubes only to the recommended volume
- Do not overfill tubes
- High g forces can drive liquid up to the cap and cause overflowing
Before using centrifuge
- Inspect the interior of the bowl for stains
- Inspect rotors and buckets for cracks and signs of corrosion
- Always check the silicon O ring for any wear & tear of the aerosol lid
- Check log book to ensure centrifuge is in good working condition
- Pre-cool centrifuge prior to use
Use of safety centrifuge
- Centrifuge load MUST always be balanced to avoid the risk of damage
- Add a balance tube with the same volume of water if processing an odd number of specimens
- Select the required time and g force or RCF
- Stop centrifuge immediately if any abnormal noise and/or shaking is noticed
- Never use the brake to stop a normal operating centrifuge for TB specimen processing
- ALWAYS open the sealed buckets inside the biosafety cabinet (BSC)
Microcentrifuge
- Always balance the rotor
- Use high-quality screw-capped tubes, which will not leak or break during centrifugation
- When using a microcentrifuge for DNA extraction, the microcentrifuge should be loaded and unloaded inside the BSC
- Remove and replace the rotor carefully
Resources
- World Health Organization laboratory biosafety manual 3rd edition, pgs 72-73
- Manufacturer's instructions
- Global Laboratory Initiative LC training module on Equipment slides 32-34.
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Each sub heading can appear…
Dr. Priyadarshini Fri, 12/01/2024 - 06:39
Each sub heading can appear in 1 slide and figures to be labelled and added in text