Smear Microscopic Examination: Different Grades of Smear Findings

Smear Microscopic Examination: Different Grades of Smear Findings

Learning Objectives

Learn to distinguish between different grades of smear findings and results, including scanty, 1+, 2+, and 3+ according to the standard classification system.

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Content

Different grades of Acid-fast Bacillus (AFB) smear microscopy and its results are as follows:

EXAMINATION	RESULT	GRADING	NO. OF FIELDS TO BE EXAMINED
More than 10 AFB per oil immersion field	Positive	3+	20
1–10 AFB per oil immersion field	Positive	2+	50
10–99 AFB per 100 oil immersion fields	Positive	1+	100
1–9 AFB per 100 oil immersion fields	Positive	Scanty (record exact number seen)	100
No AFB per 100 oil immersion fields	Negative	-	100

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