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Prevention of False Negative Results During Smear Microscopy
Learning Objectives- Learn about potential sources of error and factors contributing to false negative results in smear microscopy.
- Discuss quality assurance measures, including adherence to standard operating procedures and proficiency testing, in preventing false negative results.
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To prevent false negative results during smear microscopy, each laboratory technician needs to:
- Make sure that the sample contains sputum, not just saliva.
- Make sure there is enough sputum (at least 2-5 ml).
- Select thick, purulent particles to make the smear.
- Prepare smears correctly - not too thick, not too thin or not too little material.
- Fix the slide for the correct length of the time, not too short or too long.
- Stain with Carbol fuchsin for full five minutes.
- Do not decolorize with Sulphuric acid too intensively.
- Examine every smear for at least five minutes and full 100 fields before recording it as negative.
- Label the sputum containers, slides and laboratory forms carefully.
- Cross check the number on the laboratory form and sputum container before recording.
- Record and report the results accurately.
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