Biosafety measures required for Truenat

General biosafety requirements for Truenat laboratory include:

• Using gloves, laboratory coat, mask when handling specimens
• Minimizing aerosol generation during sample processing and handling specimens carefully
• Working in a well-ventilated room
• Using laboratory work instructions, job aids, Standard Operating Procedures (SOPs) and checklists, from sample collection to reporting results, to avoid mishaps
• Maintaining a spill management kit
• Following manufacturer instructions to operate the instruments

Precautions during processing the specimen

• Handle all specimens as potentially infectious.
• Avoid cross-contamination.
• Nozzle of the liquefaction buffer bottle should not touch the sample container.
• Avoid aerosol formation during the transfer of the liquefied sample to the lysis buffer bottle.
• Use the transfer pipettes provided with the pre-treatment pack.
• Dispose the used transfer pipettes (1% sodium hypochlorite).
• Clean the spills with 1% sodium hypochlorite, followed by 70% alcohol.

Precautions during cartridge loading and transportation

• Keep the cartridges on the provided holder while loading the specimen.
• Load the cartridges onto the machine with care.
• Avoid turning the cartridge during transportation to prevent specimen spill.
• If a spill occurs, soak tray in 1% sodium hypochlorite solution for 30 minutes and dispose. Spray the cartridge holder with 70% alcohol and load a new tray in the cartridge holder after 5 minutes.
• Pipette carefully to avoid cross-contamination between reagents and/ or samples.

Resources

• Practical Guide to Implementation of Truenat™ Tests for the Detection of TB and Rifampicin Resistance.
• Tuberculosis Laboratory Biosafety Manual.
• MolBio Diagnostics Pvt. Ltd.

Kindly provide your valuable feedback on the page to the link provided HERE