Sample Processing Control [SPC] of GeneXpert Technology

In the Cartridge Based Nucleic Acid Amplification Test (CBNAAT) machine, Sample Processing Control (SPC) monitors sample processing.

• The SPC contains non-infectious spores of Bacillus globigii. The probes and primers are provided in the cartridge to detect its target.
• These spores are co-processed with Mycobacterium tuberculosis (MTB) specimen in the cartridge by the CBNAAT machine.
• SPC control verifies:  
  • Adequate sample processing 
  • Lysis of MTB cells 
  • Amplification
  • Specimen associated inhibition 
  • Degradation of enzymes or other components of the system. 
• SPC should be positive in negative samples and can be negative or positive in positive samples.
• The result is invalid if SPC is negative in a negative sample.

Resources

• GeneXpert Dx System Operator Manual Version 4.8.

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