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CBNAAT Assay Limitations
Learning ObjectivesCBNAAT Assay Limitations
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The Cartridge-based Nucleic Acid Amplification Test (CBNAAT) test has some limitations such as:
- Bacterial load below the Limit of Detection (LOD ~ 130 CFU/ml) may result in a false-negative result.
- Patients on an anti-TB regimen can still have positive results due to killed bacilli in the specimen and hence cannot be used for follow-up.
- A positive test result does not necessarily indicate the presence of viable organisms. It is, however, presumptive for the presence of Mycobacterium tuberculosis (MTB) and Rifampicin (Rif) resistance.
- MTB detection is dependent on the number of organisms present in the sample. Quality specimen collection and timely processing of the sample will minimize the errors.
- Test results might be affected by anti-TB medication. Therefore, therapeutic success or failure cannot be assessed using this test, because DNA might persist following antimicrobial therapy.
- Mutations or polymorphisms in primer or probe binding regions may affect the detection of new or unknown MDR or Rif-resistant strains, resulting in a false-negative result.
- Any modification in sample processing may alter the performance of the test.
- Results should be interpreted in conjunction with clinical data available to the clinician.
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CBNAAT Assay Limitations
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Slide 1 can be split into 2…
Dr. Priyadarshini Mon, 25/12/2023 - 17:23
Slide 1 can be split into 2 slides