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The procedure for processing pus and other muco-purulent specimens in TB cultures is as follows:

 

  • If the specimen is thick or mucoid and less than 10 ml in volume, digest and decontaminate with N-acetyl-L-cysteine-sodium hydroxide (NALC-NaOH) method similar to the procedure used for sputum specimens.
  • If the specimen is not thick, it may be treated with 2-4% NaOH.
  • The concentration of NaOH depends upon the contaminating bacteria expected to be present in the specimen.
  • If the volume is over 10-12 ml, process only 10 ml or the first concentrate by centrifugation at 3000x g for 15-20 minutes.
    • In such a situation, if the specimen is thick, liquefy the specimen by adding a small quantity of NALC only (50-100 mg powder) and mix well.
  • After the concentration step, resuspend the sediment in 5 ml sterile water, decontaminate with NaOH and concentrate again by centrifugation.
  • Always resuspend the sediment (pellet) in buffer to reduce the pH.

 

 

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