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LPA Laboratory Equipment: Hot Air Oven
Learning ObjectivesDescribe the purpose, components, installation & maintenance of the Hot Air Oven equipment.
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Use of Hot Air Oven
- For heating non-explosive substances and objects
- For dry heat sterilization
- During DNA extraction step; kills Mycobacterium tuberculosis and partially lyses the cells at 95oC
Installation of the Hot Air Oven (Figure 1)
- The installation site must be flat and horizontal, and be able to reliably bear the weight of the appliance.
- Sufficient air circulation in the vicinity of the appliance must be guaranteed at all times.
- When installing, ensure that the distance between the wall and rear side of the appliance must be at least 15 cm.
- The clearance from the ceiling must not be less than 20 cm, and the side clearance from walls or nearby appliances must not be less than 5 cm.
Figure 1: Clearance of the hot air oven to allow sufficient air circulation
Components of Hot Air Oven (Figure 2)
- Insulated chamber surrounded by an outer case containing electric heaters
- Fan
- Shelves
- Thermocouples
- Temperature sensor
- Door locking controls
Figure 2: Hot Air Oven Components
General Instructions for Maintenance
- Always switch off the appliance before any maintenance activity.
- Always record every maintenance activity performed.
Maintenance:
- Monitor temperature
- Immediately clean up with disinfectant if there is any spill
- Leave the rack in the oven
- Lubricate the door hinges and door lock systems for proper operation
Annual Maintenance:
Performed by a qualified service technician:
- Check temperature system
- Temperature distribution
- Noise level detection
- Overall operation and alarm function
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