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Polymerase Chain Reaction (PCR) hood is a workstation enclosed by three sides.

 

Use of Polymerase Chain Reaction (PCR) Hood:

  • Used in the Master Mix Reagent Preparation and DNA Amplification Room
  • Ultraviolet (UV) light (Figure) in PCR hood denatures nucleic acids​
  • Used to decontaminate reagents and equipment prior to PCR reaction​
  • Provides protection against exogenous DNA, contaminants or inhibitors from entering the reaction tubes

 

Figure: Components of the PCR Hood

  • PCR Hood has UV light and recirculator fan with prefilters

 

  • It is made of stainless steel, therefore easier to clean

  • Always refer to the user manual for operating the equipment

General Instructions on Maintenance

  • Always switch off the appliance before any maintenance activity​.
  • Always record every maintenance activity performed​.

 

Maintenance ​

  • Clean outer and inner surfaces with freshly prepared 1% sodium hypochlorite​.
  • Replace UV bulbs and filters according to manufacturer recommendations​.

 

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