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LPA Laboratory Equipment: PCR Hood
Learning ObjectivesDescribe the uses and instructions for maintenance of the PCR Hood as a LPA Laboratory Equipment.
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Polymerase Chain Reaction (PCR) hood is a workstation enclosed by three sides.
Use of Polymerase Chain Reaction (PCR) Hood:
- Used in the Master Mix Reagent Preparation and DNA Amplification Room
- Ultraviolet (UV) light (Figure) in PCR hood denatures nucleic acids
- Used to decontaminate reagents and equipment prior to PCR reaction
- Provides protection against exogenous DNA, contaminants or inhibitors from entering the reaction tubes
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General Instructions on Maintenance
- Always switch off the appliance before any maintenance activity.
- Always record every maintenance activity performed.
Maintenance
- Clean outer and inner surfaces with freshly prepared 1% sodium hypochlorite.
- Replace UV bulbs and filters according to manufacturer recommendations.
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