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LPA Laboratory Equipment: Thermocycler
Learning ObjectivesDescribe the uses and instructions for maintenance of the Thermocycler as a LPA Laboratory Equipment.
Thermocycler is also known as Polymerase Chain Reaction (PCR) Machine.
Use of Thermocycler
Thermal cycler is used for amplification of nucleic acid by PCR.
The thermocycler should be placed in the amplification/ post-amplification area.
The features of a thermocycler are listed below.
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Features of a thermocycler:
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PCR is performed based on denaturation, amplification and annealing steps at different temperatures (Figure).
Figure: PCR Programme for MTBDR Plus Ver.2.0
General Instructions for Maintenance
- Always switch off the appliance before any maintenance activity.
- Always record every maintenance activity performed.
Annual Maintenance and Calibration
This is to be done by a qualified service engineer:
- Clean the exhaust fan assembly.
- Check all inbuilt switches.
- Temperature calibration and time validation with devices traceable to national and international standards.
- Validate the Peltier element efficiency by checking heating and cooling time, regimes and rates of block.
- Validate thermal block using PCR validation kit.
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