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LPA Laboratory Equipment: Twincubator
Learning ObjectivesDescribe the uses, features and instructions for maintenance of the Twincubator as an LPA Laboratory Equipment.
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Uses of Twincubator in Line Probe Assay (LPA):
- Different steps of the hybridization (shown in figure below) and detection procedure with DNA strips in reverse blot hybridization assays.
- In the amplification/ post-amplification area.
Figure: Twincubator used in Hybridization
Features of Twincubator:
- Provides dry incubation
- Thermo-shaker
- Programmable
- Temperature and time-controlled features
- Pre-installed temperature profile for processing of the LPA tests
- Nine storage programs with up to 20 steps
- No. of samples run - 1 to 12, including one positive and two negative controls
- User friendly with minimum risk of contamination
Note: Always refer to the user manual for operation.
Maintenance:
- Always switch off the appliance before any activity.
- Always record every maintenance activity performed.
- Clean the housing of the instrument.
- The instrument must be protected from harsh chemicals.
- Clean the instrument immediately, if liquid enters the hybridization block.
- Perform preventive maintenance (temperature check) regularly.
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