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Purpose of DNA extraction 

  • Extract DNA from cellular components 

 

For Line Probe Assay (LPA), DNA is extracted from:  

  • Clinical specimen​ 
  • Culture isolates on Loewenstein-Jensen (LJ) solid medium  
  • Bacteria growth in liquid media​ (Mycobacteria Growth Indicator Tube (MGIT), BD Diagnostics) 

 

Extraction of DNA from a clinical specimen and culture isolates (LJ, liquid) is shown in the figure below: 

Figure: Extraction of DNA from a Clinical Specimen and Culture Isolates 

 

 

 

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