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LPA PCR Reagent Preparation: Test Reagents Kit
Learning Objectives- Describe the test reagent kits Used for First Line (FL) and Second Line (SL) Line Probe Assay (LPA).
- Discuss the components of these kits.
Test Reagents Kits Used for First Line (FL) and Second Line (SL) Line Probe Assay (LPA)
- For FL LPA- kit used is MTBDR plus version 2.0
- For SL LPA- kit used is MTBDRsl version 2.0
Components of kits are shown in the following table.
|
COMPONENTS OF FL LPA KIT |
COMPONENTS OF SL LPA KIT |
|---|---|
|
Membrane strips coated with specific probes (MTBDRplus VER 2.0 STRIPS) |
Membrane strips coated with specific probes (MTBDRsl VER 2.0 STRIPS) |
|
Denaturation Solution (DEN) |
Denaturation Solution (DEN) |
|
Hybridization Buffer (HYB) |
Hybridization Buffer (HYB) |
|
Stringent Wash Solution (STR) |
Stringent Wash Solution (STR) |
|
Rinse Solution (RIN) |
Rinse Solution (RIN) |
|
Conjugate Concentrate (CON-C) |
Conjugate Concentrate (CON-C) |
|
Conjugate Buffer (CON-D) |
Conjugate Buffer (CON-D) |
|
Substrate Concentrate (SUB-C) |
Substrate Concentrate (SUB-C) |
|
Substrate Buffer (SUB-D) |
Substrate Buffer (SUB-D) |
|
Tray |
Tray |
|
Evaluation sheet |
Evaluation sheet |
|
Instructions for use |
Instructions for use |
|
Template |
Template |
|
Amplification Mix A (AM-A GT MTBDRplus VER 2) contains buffer, nucleotides, Taq polymerase |
Amplification Mix A (AM-A GT MTBDRsl VER 2.0) contains buffer, specific primers, nucleotides, Taq polymerase |
|
Amplification Mix B (AM-B GT MTBDRplus VER 2.0) contains salts, specific primers, dye |
Amplification Mix B (AM-B GT MTBDRsl VER 2.0) contains buffer, salts, dye |
All kits are stored at 2-8°C while Amplification Mix A and B (both kits) are stored at -20°C.
Resources
- GenoType MTBDR plus ver 2.0 kit, Instructions for Use.
- GenoType MTBDRsl VER 2.0 kit, Instructions for Use.
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