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Hybridization Using GT BLOT in LPA Lab Settings
Learning ObjectivesDescribe the general instructions for performing Hybridization Using GT BLOT in LPA Lab Settings.
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General Instructions
- Thoroughly clean the work bench with 1% sodium hypochlorite solution.
- Run the wash cycle A and B in GT Blot.
- After cleaning, prepare reagents as per the calculation table.
- Pour prepared reagents into reagent bottles provided with GT Blot.
- Place GT Blot reagent bottles back to machine.
- Cover the bottles with a tubed lid.
- Using clean/ new tweezers, place strips onto a clean sheet of paper and label them same as that in the work sheet.
- Use a clean/ new tray, check for any wear/ tear.
Steps for hybridization using twincubator are shown in the table below.
STEP-1 |
DISPENSE 20ΜL DENATURATION (DEN) SOLUTION IN A CORNER OF EACH WELL NOTE: NO NEED TO CHANGE TIPS |
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Step-2 |
Note: Change tips between samples |
Step-3 |
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Step-4 |
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Resources
- GenoType MTBDR plus ver 2.0 kit, Instructions for Use.
- GenoType MTBDRsl VER 2.0 kit, Instructions for Use.
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