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Contamination Control in the Hybridization Area in LPA Lab Settings
Learning ObjectivesControlling contamination in LPA lab.
H5Content
Content
Do’s and Don’ts for Contamination Control
DO’s
- Label the tools and instruments “For use in Hybridization Area”.
- Change gloves if they get contaminated amplicon.
- Decontaminate trays before reuse.
- Keep cleaned strip trays and tweezers in a clean and sealed plastic bag.
- Always clean work bench and tools with 1% freshly prepared sodium hypochlorite solution followed by 70% alcohol.
- Establish weekly laboratory cleaning protocols for floors, doors, walls.
DON’Ts
- Tools and instruments used in hybridization area should not be used elsewhere.
- Never take anything from hybridization area to reagent or specimen preparation area.
- Do not reuse falcon tubes for conjugate and substrate preparation.
- Do not decant the content of the strip tray into the sink or any other container during the Hybridisation (HYB) and Stringent (STR) steps. This will contaminate the work area.
Resources
- GenoType MTBDR plus ver 2.0 kit, Instructions for Use.
- GenoType MTBDRsl VER 2.0 kit, Instructions for Use.
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