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Do’s and Don’ts for Contamination Control 

 

DO’s

  • Label the tools and instruments “For use in Hybridization Area”.​
  • Change gloves if they get contaminated amplicon.​
  • Decontaminate trays before reuse​.
  • Keep cleaned strip trays and tweezers in a clean and sealed plastic bag.​
  • Always clean work bench and tools with 1% freshly prepared sodium hypochlorite solution followed by 70% alcohol​.
  • Establish weekly laboratory cleaning protocols for floors, doors, walls.

 

DON’Ts

  • Tools and instruments used in hybridization area should not be used elsewhere​.
  • Never take anything from hybridization area to reagent or specimen preparation area​.
  • Do not reuse falcon tubes for conjugate and substrate preparation.​
  • Do not decant the content of the strip tray into the sink or any other container during the Hybridisation (HYB) and Stringent (STR) steps. This will contaminate the work area​.

 

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