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Evaluation of Second-line Line Probe Assay Strip
Learning Objectives- Describe the key components of the SL - LPA Evaluation Strip.
- Key considerations during the evaluation of an LPA strip.
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Each strip for Second Line - Line Probe Assay (SL - LPA) has a total of 27 reaction zones (Figure below).
Figure: Key Zones of SL - LPA Evaluation Strip; Source: GenoType MTBDRsl VER 2.0 kit, Instructions for Use.
Evaluation of Assay Strip for SL- LPA Zones:
- Evaluation of Conjugate Control (CC), Amplification Control (AC), M. tuberculosis complex (TUB) are the same as described for First Line - LPA.
- Locus Controls (gyrA, gyrB, rrs, eis)
- Detect gene region specific (gyrA/ gyrB/ rrs/ eis)
- Wild type probes
- Comprise the resistance regions of respective genes (gyra/ gyrb/ rrs/ eis)
- Absence of signal for at least one of the wild-type probes indicates resistance of the tested strain to the respective antibiotic
- Mutation probes
- Detect most common resistance-mediating mutations (gyra/ gyrb/ rrs/ eis)
- Each pattern deviating from the wild type pattern indicates resistance
Important: For an accurate evaluation, align provided template separately for each locus with the respective Locus Control band.
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