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xamples of banding patterns for First Line - Line Probe Assay (FL - LPA) are shown in figure below.

 

Description

  • All wild type bands display signal, mark as “+” against Wild Type (WT) column of the respective gene
  • If at least one of the wild type bands is absent, mark as “-“ against WT column
  • Similarly, “+” or “-” for Mutation (MUT) probes

Figure: Banding patterns examples for Rifampicin and Isoniazid resistance; Source: GenoType MTBDR plus ver 2.0 kit, Instructions for Use.

 

 

​Example #1

  • The wild type banding pattern
  • All wild type probes, but none of the mutation probes display a signal; hence, the evaluation chart shows “+”
  • Three wild type columns and “–” in three mutation columns; hence “rifampicin (rmp) sensitive” and “Isoniazid (INH) sensitive” are marked “+”

 

Example #5

  • One rpob and katg wild type probes missing; hence, the boxes for “rpob WT” and “katg WT” are marked with “–”
  • No mutation probes developed; boxes are marked “–”
  • inhA promoter region does not deviate from the wild type pattern, hence strain is evaluated as resistant to RMP and INH

 

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