Content Status

Type

Linked Node

H5Content
Content

Reporting Drug-resistance in Special Cases

 

  • When specimen tested contains hetero resistant strain:
    • Strips contain both wild type and mutant probes for three genes known for resistance to Rifampicin and Isoniazid
      • Bands to be interpreted as indicating resistance to a particular drug; should be reported as such
      • Example of hetero resistant strain: rpoB WT 8 and rpoB MUT 3 bands are present
  • The tested specimen contains more than one Mycobacterium tuberculosis complex strain (due to mixed culture/ non-tuberculous mycobacterium or contamination):
    • If any strains harbor mutation, one mutation probe, as well as the corresponding wild type probe, may stain positive; ascertain the presence of TUB band
    • Repeat test or phenotypic testing to confirm resistance
  • Presence of non-tuberculous mycobacterium:
    • TUB band missing; not be reported as M. tuberculosis
    • TUB band absent but random banding patterns visible, eg: bands at rpoB WT 1, 4, 5 or 8; not be reported as M. tuberculosis

 

Resources

 

 

Kindly provide your valuable feedback on the page to the link provided HERE
 

Content Creator

Reviewer