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Interpretation of SL-LPA: Interpretation Examples
Learning Objectives-
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he examples of banding patterns for Second Line - Line Probe Assay (SL - LPA) are shown in the figure below.
Description
- All wild type bands display signal, mark as “+” against Wild Type (WT) column of the respective gene.
- If at least one of the wild type bands is absent, mark as “-“ against WT column. Similarly, “+” or “-” for Mutation probes (MUT).
Figure: Examples for banding patterns for SL - LPA; Source: GenoType MTBDRsl Ver 2.0 Kit, Instructions for Use.
Example #1
- The wild type banding pattern.
- All wild type probes, but none of the mutation probes display a signal; hence, evaluation chart shows “+”.
- Four wild type columns and “–” in the four mutation columns; hence, no entry made in the fields of resistance columns.
Example #7
- gyrA locus and gyrB locus show wild type pattern; hence, evaluation chart shows “+” for fluoroquinolone (ofloxacin) resistance.
- rrs locus shows wild type pattern; hence, evaluation chart shows “+” for Kanamycin.
- eis wild type bands are present and “eis MUT” is absent; low-level Kanamycin resistance is assigned to the test.
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