LPA Troubleshooting for Other Possible Reasons

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Issue #1:

Absence of Conjugate Control (CC) and Amplification Control (AC) in a negative control (Figure 1).

Figure 1: Absence of CC and AC in a Negative Control

Table 1 below lists the reasons and troubleshooting for the absence of CC and AC in a negative control.

Table 1: Reasons and troubleshooting for the absence of CC and AC in a Negative Control
REASONS	TROUBLESHOOTING
Mistakes during setup	The test is invalid – Repeat test
Performance of amplification reaction
Presence of amplification inhibitors

Issue 2:

Absence of locus control for a specific drug, with presence of CC and M. tuberculosis complex (TUB) band (Figure 2).

Figure 2: Absence of locus control for a specific drug, with presence of CC and TUB band

Table 2 below lists the reasons and troubleshooting for the absence of locus control for a specific drug, with the presence of CC and TUB band.

Table 2: Reasons and troubleshooting for the absence of locus control for a specific drug, with the presence of CC and TUB band
REASONS	TROUBLESHOOTING
Mutation or deletions in locus control region	The test is indeterminant - Repeat test Sequencing may be requested to identify the specific mutation
Complete or partial deletion of a target gene

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