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LPA Quality Assurance: Cleaning of Lab
Learning Objectives-
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General Instructions
- Dedicated cleaning supplies, brooms required for different areas.
- Cleaning should be done by trained Polymerase Chain Reaction (PCR) laboratory staff, not by general cleaning personnel.
- Meticulous cleaning of the work area, surfaces, racks, pipettes and instruments should be done before and after performing each procedure with disinfectants (freshly prepared 1% sodium hypochlorite, followed by 70% alcohol).
- Use dedicated spray flasks/ containers for disinfectants.
- Clean paper towels (not cloth) should be used to decontaminate surfaces and equipment.
- Walls should have high-gloss enamel/ gloss-based paint for easy cleaning.
- Floors must be covered with vinyl, linoleum or ceramic tiles.
- Bench-tops must be made of impermeable melamine to eliminate crevices.
- Instruments should be cleaned and maintained daily/ weekly as described in the equipment training content.
- Cleaning and maintenance log sheets of all areas should be maintained.
Area-wise Cleaning
Weekly meticulous cleaning once; clean top-to-bottom, starting with bench tops and finishing with mopping the floor with disinfectants.
Area 1: DNA extraction room
Clean and maintain instruments (pipettes, biosafety cabinet, centrifuge and water bath) daily/ weekly.
Area 2: Pre-amplification room
Clean and maintain instruments (pipettes, PCR equipment) daily/ weekly.
Area 3: Amplification room
Clean and maintain instruments (thermal cycler, pipettes, PCR equipment) daily/ weekly.
Area 4: Hybridization room
Clean and maintain instruments (GT-Blot, Twincubator, pipettes, GT-Blot hybridization machine) daily/ weekly.
Resources
- Line Probe Assays for Drug-resistant Tuberculosis Detection, GLI.
- Molecular Detection of Drug-resistant Tuberculosis by Line Probe Assay, FIND.
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