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General Instructions

 

  • Dedicated cleaning supplies, brooms required for different areas.
  • Cleaning should be done by trained Polymerase Chain Reaction (PCR) laboratory staff, not by general cleaning personnel.
  • Meticulous cleaning of the work area, surfaces, racks, pipettes and instruments should be done before and after performing each procedure with disinfectants (freshly prepared 1% sodium hypochlorite, followed by 70% alcohol).
  • Use dedicated spray flasks/ containers for disinfectants.
  • Clean paper towels (not cloth) should be used to decontaminate surfaces and equipment.
  • Walls should have high-gloss enamel/ gloss-based paint for easy cleaning.
  • Floors must be covered with vinyl, linoleum or ceramic tiles.
  • Bench-tops must be made of impermeable melamine to eliminate crevices.
  • Instruments should be cleaned and maintained daily/ weekly as described in the equipment training content.
  • Cleaning and maintenance log sheets of all areas should be maintained.

 

Area-wise Cleaning

 

Weekly meticulous cleaning once; clean top-to-bottom, starting with bench tops and finishing with mopping the floor with disinfectants.

 

Area 1: DNA extraction room

Clean and maintain instruments (pipettes, biosafety cabinet, centrifuge and water bath) daily/ weekly.

 

Area 2: Pre-amplification room

Clean and maintain instruments (pipettes, PCR equipment) daily/ weekly.

 

Area 3: Amplification room

Clean and maintain instruments (thermal cycler, pipettes, PCR equipment) daily/ weekly.

 

Area 4: Hybridization room

Clean and maintain instruments (GT-Blot, Twincubator, pipettes, GT-Blot hybridization machine) daily/ weekly.

 

 

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