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Moderate-risk TB Laboratories
Learning Objectives-
H5Content
Content
Moderate-risk TB Laboratories can:
- Process specimens for inoculation on primary solid-culture media
- Perform direct line-probe assays and direct Drug Sensitivity Testing (DST)
Essential Minimum Biosafety Measures
- Biological Safety Cabinets (BSCs): Class I or II
- Ventilation: 6-12 ACH
- Personal Protective Equipment: Laboratory gowns and gloves
- Laboratory design: Restricted traffic area
- Decontamination and waste disposal
- Procedures optimized for minimizing the generation of aerosols
Challenges of Moderate-risk TB Laboratories
- Staff may work in areas with poor ventilation and/ or poor illumination.
- BSCs may be poorly maintained and not certified or not be properly ducted.
- Careless manipulation of specimens may lead to aerosolization.
- Specimen containers may break or leak during centrifuging.
- Adequate warnings of biohazards may be lacking, and information on who should be contacted during an emergency may be inadequate.
- Cooling or heating systems may not work properly.
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