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The following are the measures to be kept in mind when adding immersion oil to the slide:

 

  • Before adding immersion oil to the slide, ensure that the smear is facing upwards (Figure 1).

Figure 1: Slide should Face Upwards when Mounted on the Microscope Stage

 

  • Add a drop of immersion oil, using the applicator (dropper bottle).
  • The drop must fall freely onto the smear, so that the oil applicator does not touch the slide and get contaminated (Figure 2).

Figure 2: Oil Dropped onto Slide with an Applicator

 

  • Mount the slide on the microscope and use the 10X objective lens to focus the smear, scan and look for mucoid material.
  • Carefully rotate the 100X objective lens over the slide, adjust, sharp focus and observe under immersion oil.

 

Do’s and Don’ts:

  • The 100X objective is the only lens requiring immersion oil.
  • Keep the immersion oil away from other lenses.
  • Use a good quality immersion oil.
  • The immersion oil must have a medium viscosity and a refractive index (RI) greater than 1.5.
  • Never allow the oil applicator to touch the slide.
  • Do not use cedar wood oil diluted with xylene as it leaves a sticky residue on the lens and destroys the lens over time. 

 

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