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Errors 5006, 5007, 5008 and 5009 (Probe Check Control Failed) sometimes appear on the Cartridge-based Nucleic Acid Amplification Test (CBNAAT) machine in TB laboratories and need troubleshooting.

Problem:

Probe Check Control (PCC) failed, and the test was stopped before amplification.

Origins:

  • Incorrect storage of cartridges (probe integrity issues detected)
  • Dust on optical filters
  • Sample too viscous
  • Incorrect sample volume
  • Improper fluid transfer (bubbles)

Solutions:

  • Store the kits between 2 - 28°C.
  • Use an optical brush to clean the optical filters (without a cleaning solution).
  • Make sure the sample is totally liquified before transferring it to the cartridges.
  • Add the correct volume of the specimen.
  • Avoid making bubbles.

 

Video file

Video : CBNAAT Troubleshooting: Error- 5006, 5007, 5008 and 5009 [PCC Failed]

 

Video file

Video : Probe Check Control (PCC)

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