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Disinfectants used in lab settings include:

1% Sodium Hypochlorite

  • Broad spectrum antimicrobial action
  • Used to disinfect surfaces
  • Used to disinfect infectious material and disposal of used Truenat consumables (reagent bottles, cartridges, tips, chips) 
  • Hazardous and corrosive, to be used with care
  • Is highly alkaline so can corrode metal
  • Waste soaked in Sodium Hypochlorite should not be discarded by autoclaving. 

 

70% Alcohol

  • Bactericidal action
  • Used for surface decontamination only
  • Highly inflammable; keep away from fire
  • Used to disinfect biosafety cabinets, laboratory benches and surface of instruments.

 

5% Phenol

  • Used for decontaminating Cartridge-based Nucleic Acid Amplification Testing (CBNAAT) equipment and single-use items like CBNAAT cartridges prior to disposal
  • Highly irritating to the skin, eyes and mucous membranes.

 

Preparation of these disinfectants is described below.

 

Preparation of 1% Sodium Hypochlorite

  • Use commercially available 4% sodium hypochlorite solution.
  • Dilute with distilled water to prepare required amount of 1% sodium hypochlorite
    • E.g.: To prepare 100 ml of 1% sodium hypochlorite: 75 ml distilled water plus 25 ml 4% sodium hypochlorite solution.
  • Sodium hypochlorite solutions (domestic bleach) contain 50 g/l available chlorine, and should therefore be diluted to 1:50 or 1:10 in water to obtain the final concentrations of 1 g/l or 5 g/l when used as a general-purpose disinfectant for TB laboratories.
  • To be prepared fresh.

 

Preparation of 70% Alcohol

  • Use commercially available absolute alcohol.
  • Dilute with distilled water to prepare the required amount of 70% alcohol
    • E.g.: To prepare 100 ml of 70% alcohol: 70 ml absolute alcohol plus 30 ml distilled water.

 

Preparation of 5% Phenol

  • Melt 5 g of phenol by heating it.
  • Dissolve in 100 ml distilled water.
Video file

Video : Preparation of TB Lab Disinfectants

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