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Preparation of TB Lab Disinfectants
Learning ObjectivesPreparation of TB Lab Disinfectants
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Disinfectants used in lab settings include:
1% Sodium Hypochlorite
- Broad spectrum antimicrobial action
- Used to disinfect surfaces
- Used to disinfect infectious material and disposal of used Truenat consumables (reagent bottles, cartridges, tips, chips)
- Hazardous and corrosive, to be used with care
- Is highly alkaline so can corrode metal
- Waste soaked in Sodium Hypochlorite should not be discarded by autoclaving.
70% Alcohol
- Bactericidal action
- Used for surface decontamination only
- Highly inflammable; keep away from fire
- Used to disinfect biosafety cabinets, laboratory benches and surface of instruments.
5% Phenol
- Used for decontaminating Cartridge-based Nucleic Acid Amplification Testing (CBNAAT) equipment and single-use items like CBNAAT cartridges prior to disposal
- Highly irritating to the skin, eyes and mucous membranes.
Preparation of these disinfectants is described below.
Preparation of 1% Sodium Hypochlorite
- Use commercially available 4% sodium hypochlorite solution.
- Dilute with distilled water to prepare required amount of 1% sodium hypochlorite
- E.g.: To prepare 100 ml of 1% sodium hypochlorite: 75 ml distilled water plus 25 ml 4% sodium hypochlorite solution.
- Sodium hypochlorite solutions (domestic bleach) contain 50 g/l available chlorine, and should therefore be diluted to 1:50 or 1:10 in water to obtain the final concentrations of 1 g/l or 5 g/l when used as a general-purpose disinfectant for TB laboratories.
- To be prepared fresh.
Preparation of 70% Alcohol
- Use commercially available absolute alcohol.
- Dilute with distilled water to prepare the required amount of 70% alcohol
- E.g.: To prepare 100 ml of 70% alcohol: 70 ml absolute alcohol plus 30 ml distilled water.
Preparation of 5% Phenol
- Melt 5 g of phenol by heating it.
- Dissolve in 100 ml distilled water.
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Video : Preparation of TB Lab Disinfectants
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