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Comparing AFB and LED-Fluorescence Microscopy
Learning ObjectivesComparing AFB and LED-Fluorescence Microscopy
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The comparison of the Acid-Fast Bacilli (AFB) and Light Emission Diode (LED) Fluorescence Microscopy is tabulated below:
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Direct Microscopy for Acid Fast Bacilli (AFB)
Figure 1: Bright Field Microscope used in Ziehl–Neelsen (ZN) staining of AFB |
Light Emission Diode (LED) Fluorescence Microscopy
Figure 2: LED-Fluorescent Microscope used in fluorescent staining of AFB |
|
Figure 3: ZN-stained slide used in direct microscopy |
Figure 4: Auramine-O-stained slide used in fluorescent microscopy |
| Method used widely for the diagnosis and confirmation of pulmonary tuberculosis | Method used less commonly for the diagnosis and confirmation of pulmonary tuberculosis |
| Staining procedure complex | Staining procedure simpler |
| Examination at lower magnification is NOT possible | Examination at lower magnification is possible |
| Takes more time than LED-FM | Takes 75% less time than ZN and chances to lose scanty slides are also minimized. |
| Low durability | High durability |
| Less sensitive | 10% more sensitivity than Bright Field ZN microscopy |
| As specific as LED-FM | Equally as specific as ZN microscopy |
| No need for an experienced lab technician | Needs an experienced lab technician |
| Technical errors are common | Technical errors are less common |
| Misses the paucibacillary TB cases, especially when the patient is co-infected with HIV | Identifies the paucibacillary TB cases especially when the patient is co-infected with HIV |
Resources
- WHO Policy Statement - Fluorescence Light Emitted Microscope for the Diagnosis of TB, 2010.
- A Comparative Study of Auramine Staining using LED Fluorescent Microscopy with Ziehl- Neelsen Staining in the Diagnosis of Pulmonary Tuberculosis, Journal of Evolution of Medical and Dental Sciences, Volume 2, Issue 20, May 2013.
- Comparison of Direct versus Concentrated Smear Microscopy in Detection of Pulmonary Tuberculosis, BMC Res Notes, 2013; 6 : 291.
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