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Steps to be done after Culture Specimen Processing
Learning ObjectivesLearn about Steps to be done after Culture Specimen Processing.
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After specimen processing, the resuspended pellet may be used for various tests as per the laboratory policy or national guidelines recommended for TB diagnosis such as:
- Inoculation of sediments in Mycobacterium Growth Indicator Tube (MGIT) broth (Liquid media) and or solid media for primary culture isolation
- Subculture a loopful on a Brain Heart Infusion (BHI) Agar plate for contamination check
- Concentrated smear of processed specimen can be prepared if not prepared earlier, for a microscopy smear
- DNA extraction for Line Probe Assay
Transfer the remaining sediment into 2 mL labelled cryovials and store at 2-8⁰C for repeat or additional testing.
Figure: Steps After Sample Processing
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