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The Truenat Assay Technology works on Real-time Polymerase Chain Reaction (PCR).

 

The key steps include:

  1. Collection of specimens from a presumptive TB/known TB Patient (for UDST).
  2. Liquefaction and lysis of specimen using the Trueprep AUTO MTB sample pre-treatment pack (20 minutes)
  3. Extraction and purification of DNA using Trueprep AUTO v2 Universal Cartridge-based sample prep kit and Trueprep AUTO v2 Universal Cartridge-based sample prep device (20 minutes)
  4. Amplification of extracted DNA by the Truelab Real-time micro-PCR analyzer using freeze dry PCR reagent in microtubes on a Truenat MTB chip (35 minutes)
  5. DNA from positive test results is tested using Truenat MTB RIF Dx chip as a reflex test (55 minutes)

 

The process flow from sample to result for Truenat Assay Technology is described in the figure below.

 

Figure: Process flow from sample to result for Truenat Assay Technology; Source: Practical Guide to Implementation of Truenat™ Tests for the Detection of TB and Rifampicin Resistance.

 

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