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Procedure for Culture Specimen Processing: Bronchial Lavage
Learning ObjectivesProcedure for Culture Specimen Processing: Bronchial Lavage
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The procedure for processing bronchial lavage specimens in TB cultures is the same as that for sputum samples. The following aspects need to be considered:
- If the specimen is larger than 10 ml in volume, it needs to be concentrated by centrifugation (3000x g, 15-20 minutes) and the sediment resuspended in 5 ml sterile water.
- Thick/ mucoid samples need to be liquefied by mixing the specimen with a pinch (50-100 mg) of N-acetyl-L-cysteine (NALC) powder.
The processed specimen is used to inoculate the MGIT tubes.
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