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Procedure for Culture Specimen Processing: Laryngeal Swab
Learning ObjectivesProcedure for Culture Specimen Processing: Laryngeal Swab
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The procedure for processing laryngeal swab specimens in TB cultures is as follows:
- Transfer the swab (without a stick) into a sterile centrifuge tube and add 2 ml sterile water.
- Add 2 ml N-acetyl L-cysteine - sodium hydroxide (NALC-NaOH) solution, replace the cap, mix well (vortex mix), keep for 15 minutes.
- Remove the swab with forceps, squeezing the liquid out of the swab, and discard the swab.
- Fill the tube with phosphate buffer, mix and centrifuge at 3000x - 3500x g (15-20 minutes).
- Discard the supernatant fluid and resuspend the sediment in 1-2 ml sterile buffer.
The processed specimen is used to inoculate the MGIT tubes.
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