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Procedure for Culture Specimen Processing: Tissue
Learning ObjectivesProcedure for processing tissue specimens in TB cultures
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The procedure for processing tissue specimens in TB cultures is as follows:
- Add saline or water (2 - 4 ml) and homogenize the tissue in a Biosafety Cabinet (BSC) using sterile equipment:
- Tissue grinder or homogenizer
- If these are not available, use a mortar and pestle
- Small tissue specimens may be placed in a petri dish with 2-4 ml sterile water and torn apart with the help of two sterile needles
- Decontaminate the homogenized specimen with N-acetyl L-cysteine - sodium hydroxide (NALC-NaOH) procedure as in processing sputum. Tissue biopsies collected aseptically do not require decontamination procedures.
- Resuspend the sediment in phosphate buffer.
The processed specimen is used to inoculate the MGIT tubes.
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