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Biosafety Requirements in Line Probe Assay [LPA] Laboratory
Learning ObjectivesBiosafety Requirements in Line Probe Assay [LPA] Laboratory
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DNA extraction for Line Probe Assay (LPA) from clinical specimens can be performed in either a BSL-2 or BSL-3 laboratory, while DNA extraction from mycobacterial cultures must be performed in a BSL-3 laboratory. Only after heat-killing of the organism and DNA isolation can the sample be considered non-infectious and moved to the LPA laboratory.
The subsequent steps, i.e., amplification and post-amplification, only require a BSL-1 laboratory.
Aspects of biosafety in LPA laboratory include:
- Facility Design
- Workflow description
- Rooms required to complete LPA procedures
- Access to authorized personnel only
- Electricity and backup power supply
- Laboratory Equipment
- Equipment operation
- Equipment maintenance
- Safe Lab Practices
- Cleaning and disinfecting work areas
- Use of Personal Protective Equipment (PPE)
- Disinfection and decontamination
- Biomedical waste management
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