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Equipment Required in Different Rooms in the LPA Lab Facility
Learning ObjectivesEquipment Required in Different Rooms in the LPA Lab Facility
The design of the LPA facility includes the availability of different rooms to perform different steps. Each room has a set of specific equipment.
There should be a minimum of 4 separate rooms to carry out procedures. The details of these rooms and equipment are elaborated below:
1. DNA Extraction Room: For DNA extraction from decontaminated samples. Table 1 shows the equipment in the DNA extraction room.
BIOSAFETY CABINET | MICROLITER CENTRIFUGE | VORTEX MIXER |
---|---|---|
|
|
|
HOT AIR OVEN | REFRIGERATOR | |
|
|
2. Master Mix/ Reagent Preparation Room: For the preparation of reagents required in the master mix for PCR. Table 2 shows the equipment required in this room.
PCR HOOD | PICO-FUGE | -20ºC FREEZER |
---|---|---|
3. DNA Amplification Room: For PCR amplification of DNA. Table 3 shows the equipment in this room.
PCR HOOD | THERMAL CYCLER | REFRIGERATOR |
---|---|---|
4. Hybridization – detection Room: For hybridization of amplicons with probes and detection of bands. Table 4 shows the equipment required in this room.
TWINCUBATOR | GT BLOT |
---|---|
REFRIGERATOR | WATER BATH |
Resources
- Molecular Detection of Drug-resistant Tuberculosis by Line Probe Assay.
- GLI Training Package on LPA.
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