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The design of the LPA facility includes the availability of different rooms to perform different steps. Each room has a set of specific equipment.

 

There should be a minimum of 4 separate rooms to carry out procedures. The details of these rooms and equipment are elaborated below:

 

1. DNA Extraction Room: For DNA extraction from decontaminated samples. Table 1 shows the equipment in the DNA extraction room.

 

Table 1: Equipment in the DNA Extraction Room
BIOSAFETY CABINET MICROLITER CENTRIFUGE VORTEX MIXER

 

 

 

HOT AIR OVEN REFRIGERATOR  

 

 

 

 

2. Master Mix/ Reagent Preparation Room: For the preparation of reagents required in the master mix for PCR. Table 2 shows the equipment required in this room.

 

Table 2: Equipment in the Master Mix/Reagent preparation room
PCR HOOD PICO-FUGE -20ºC FREEZER

 

3. DNA Amplification Room: For PCR amplification of DNA. Table 3 shows the equipment in this room.

 

Table 3: Equipment in the DNA Amplification Room
PCR HOOD THERMAL CYCLER REFRIGERATOR

 

4. Hybridization – detection Room: For hybridization of amplicons with probes and detection of bands. Table 4 shows the equipment required in this room.

 

Table 4: Equipment in the Hybridization-detection Room
TWINCUBATOR GT BLOT
REFRIGERATOR WATER BATH

 

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