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The Truenat is a rapid molecular diagnostic method based on the Nucleic Acid Amplification Testing (NAAT) principle. Truenat uses portable, battery-operated devices to rapidly detect Mycobacterium tuberculosis Complex Bacteria (MTBC) and Rifampicin resistance.

Truenat system involves two main devices:

  1. Trueprep® AUTO v2 Universal Cartridge based Sample Prep Device: Used for the automated extraction and purification of DeoxyriboNucleic Acid (DNA).
  2. Truelab® Real Time micro-PCR Analyzer: Used for performing Real-Time Polymerase Chain Reaction (RT-PCR), resulting in the semi-quantitative detection of MTBC.

The errors in Truenat are broadly categorised as:

  • Errors with codes (Trueprep and Truelab errors)
  • Errors without codes (System failures)

The errors without codes in Truenat and the ways to resolve them are listed in the table below:

Sl. No. System failures Reason Troubleshoot
1 ‘Unable to read chip information’ Analyzer was unable to read chip memory Check if the chip was loaded properly into the tray. Remove the chip and re-select the profile from Status Screen and repeat the steps. If the message reappears, load a new chip and re-load the elute again
2 ‘Could not initialize. Please try again’ The system was unable to establish an internal connection Attempt the test again by using a new chip and re-loading the elute again
3 “Chip is already used” OR “Chip loaded is expired” User loaded a used chip/expired chip in the tray Use a fresh chip and re-load the elute
4 Login attempt failure Login failed due to incorrect password entered Re-enter the correct password
5 Invalid patient name in sample details screen The patient’s name in the sample details section is blank Fill patient’s name in the sample details section

 

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Question    

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Answer 2    

Answer 3    

Answer 4    

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Correct explanation    

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Part of Post-test    

What should you do when you get a system failure alert as: 

“ Chip is already used” ?

Re-insert the same chip

Insert a fresh chip

Do not insert any chip

None of the above

    2

When you get a system failure alert as: “ Chip is already used”  , Use a fresh chip and re-load the elute.

    

   Yes

 Yes

What should you do when you get a system failure alert as:

"Unable to read chip information"

Re-insert the same chip

Insert a fresh chip

Do not insert any chip

Check if chip is loaded properly, remove the chip and reselect the profile from status screen and repeat the steps. 

4 When you get a system failure alert as "Unable to read chip information" Check if the chip was loaded properly into the tray. Remove the chip and re-select the profile from Status Screen and repeat the steps. If the message reappears, load a new chip and re-load the elute again  

 

Yes

 

Yes

 

 

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