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Storing a sputum sample
Learning ObjectivesThe learner will be able to describe the storage conditions for a sputum sample for microscopy, molecular tests and liquid culture.
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Storage conditions of sputum sample can effect the test results.
- Sputum samples should be transported to the laboratory as soon as possible after collection.
- It is the responsibility of Laboratory Technician (LT) and Senior TB Laboratory Supervisor (STLS) to ensure proper storage and transport of sputum specimens.
- Sputum is stored to preserve the specimen quality.
- The stored sputum samples should not be frozen.
Storage of Sputum Samples
For microscopy
- For smear microscopy, sputum specimens should be examined on the same day and not later than 2 days after collection.
- If delay is unavoidable, the sputum collected should be stored in a cool place/ refrigerated at 4°C to inhibit the growth of unwanted microorganisms.
- Stored sputum samples should be protected from light and heat to prevent liquefaction of the sample, else it makes the selection of mucopurulent part of the sample difficult.
- Samples received over holidays/weekends should be stored in a cool place/ refrigerated at 4°C.
For liquid culture
- Sputum should be stored in a cool place/ refrigerated at 4°C to inhibit the growth of unwanted microorganisms; not later than 3 days after collection.
For molecular tests - Nucleic Acid Amplification Test (NAAT) and Line Probe Assay (LPA)
- Sputum must be stored by refrigerating at 4°C to inhibit the growth of unwanted microorganisms and transported in cool chain to the nearest molecular laboratory. It should not be stored beyond one week at 4°C.
Resources
Technical and Operational Guidelines; Chapter 3: Case finding and diagnosis strategy
Assessment
Question | Answer 1 | Answer 2 | Answer 3 | Answer 4 | Correct answer | Correct explanation | Page id | Part of Pre-test | Part of Post-test |
The sputum collected expecting a time delay for processing should be stored in cool place/ refrigerated at 4°C. | True | False | 1 |
The sputum collected expecting a time delay for processing should be stored in cool place/ refrigerated at 4°C.
|
Yes | Yes |
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