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After air drying and heat-fixing of the smear, it needs to be stained.

Fluorescence microscopy (FM) staining should always be carried out in a designated area. The reagents required for the FM staining procedure are shown in Figure 1. The steps involved in the staining procedure are shown in Figure 2.

 

Figure 1: Reagents Required for FM Staining

 

Figure 2: Steps involved in FM staining procedure

 

For a visual representation of the above-mentioned steps, please click the video below:

Video file

 

Resources

Laboratory Diagnosis by Sputum Smear Microscopy, GLI Initiative

 

Assessment

Question Answer 1 Answer 2 Answer 3 Answer 4 Correct answer Correct explanation Page id Part of Pre-test Part of Post-test
What is the counterstain used in FM staining? Acid Alcohol Potassium Permanganate Phenol Auramine-O 2 In FM staining, Potassium Permanganate is used as the counterstain.   Yes Yes

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