{"presentation":{"slides":[{"elements":[{"x":3.2679738562091507,"y":6.490280777537798,"width":94.77124183006536,"height":93.52051835853132,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"

Procedure:<\/strong><\/span><\/p>\n\n

Equipment and reagents<\/strong><\/span><\/p>\n\n

\n\t
Truelab Uno\/ Duo\/ Quattro Real Time Quantitative micro\u2013PCR analyzer<\/li>\n\t
Truenat MTB micro-PCR kit<\/li>\n\t
Truenat MTB Rif micro-PCR kit<\/li>\n\t
Truepet fixed volume (6 \u03bcl) precision micropipette<\/li>\n<\/ul>\n\n
<\/p>\n\n
Amplification and detection of <\/strong>Mycobacterium tuberculosis <\/strong><\/em>(MTB)<\/strong><\/span><\/p>\n\n
\n\t
Wear gloves, laboratory coats, mask.<\/li>\n\t
Clean working surfaces with 1% Sodium hypochlorite, followed by 70% alcohol.<\/li>\n\t
Clean instruments with a paper towel dipped in 70% alcohol.<\/li>\n<\/ol>\n"},"subContentId":"0acfb703-ccb5-49b7-b033-618ae1904fe2","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":3.2679738562091503,"y":19.43844492440605,"width":21.78649237472767,"height":0.2159827213822894,"action":{"library":"H5P.Shape 1.0","params":{"line":{"borderWidth":"1","borderStyle":"dotted","borderColor":"#000"},"type":"horizontal-line","shape":{"fillColor":"#ffffff","borderColor":"#000000","borderWidth":"0","borderStyle":"solid","borderRadius":"0"}},"subContentId":"ab24fe7f-4522-4bad-a0d7-90973f189acf","metadata":{"contentType":"Shapes","license":"U"}},"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false},{"x":4.357298474945534,"y":69.11447084233261,"width":57.734204793028326,"height":0.2159827213822894,"action":{"library":"H5P.Shape 1.0","params":{"line":{"borderWidth":"1","borderStyle":"dotted","borderColor":"#000"},"type":"horizontal-line","shape":{"fillColor":"#ffffff","borderColor":"#000000","borderWidth":"0","borderStyle":"solid","borderRadius":"0"}},"metadata":{"contentType":"Shapes","license":"U"},"subContentId":"b408e414-44c2-44e5-b664-2fbf4abae299"},"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false}],"slideBackgroundSelector":{}},{"elements":[{"x":2.178649237472767,"y":6.479481641468682,"width":92.5925925925926,"height":86.39308855291577,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
4. Switch on the Truelab device by pressing the red button for two seconds.<\/p>\n\n
5. Select test bay.<\/p>\n\n
6. Select test profile: \u201cMTB\u201d, enter Details (Referred By, Patient ID, Gender, Patient Name, Age).<\/p>\n\n
7. Select the sample type.<\/p>\n\n
8. Press \u201cStart Reaction\". For Truelab Uno Dx, Press the eject button to open the chip tray. For Truelab Duo\/Quattro, the chip tray opens automatically on tapping the \u201cStart Reaction\u201d .<\/p>\n\n
9. Take out \u201cTruenat MTB chip\u201d from the chip sleeve (check desiccant colour. If blue -chip can be used; discard chip if desiccant is pink\/ white which indicates moisture is absorbed).<\/p>\n\n
10. Place the chip on the tray by aligning the registration holes with tray pins without touching the white reaction well. (the white reaction well should face upward).<\/p>\n\n
11. Open the microtube containing the freeze-dried PCR reagent (white colour) and place it on the microtube stand.<\/p>\n"},"subContentId":"30cf382b-d58e-45a5-9c14-4fd0ae2a48e8","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""}],"slideBackgroundSelector":{}},{"slideBackgroundSelector":{},"elements":[{"x":2.178649237472767,"y":15.11879049676026,"width":91.50326797385621,"height":66.95464362850971,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
12. Pipette 6 \u03bcl DNA from the Extraction Chamber (After DNA extraction and purification step) into the microtube.<\/p>\n\n
13. Wait 30-60 seconds for the DNA elute and PCR reagent to mix (clear solution obtained).<\/p>\n\n
14. Use the same tip to pipette 6 \u03bcl treated DNA from the microtube and load on the white reaction well of the chip.<\/p>\n\n
15. Discard the microtube and microtip (1% sodium hypochlorite).<\/p>\n\n
16. Start the test run.<\/p>\n\n
17.The test completes in 35 minutes, press \u2018\u2019RESULT\u2019\u2019 to view the result screen\\<\/p>\n\n
<\/p>\n"},"subContentId":"7c426ed1-92da-48f5-a403-8c5ed3872590","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""}]},{"elements":[{"x":3.2679738562091507,"y":8.663606911447083,"width":94.77124183006536,"height":71.27429805615552,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
<\/p>\n\n
18. Possible results<\/p>\n\n
\n\t
MTB Detected\/ Not Detected\/ Errors\/ Invalid<\/li>\n<\/ul>\n\n
19. If MTB detected, test the same DNA eluate for Rif resistance using MTB-Rif chip. Select the MTB-Rif assay.<\/p>\n\n
20. If the result is Invalid\/ Error, repeat amplification using the same extracted DNA and a new \u201cTruenat MTB chip\u201d. If Invalid again, repeat the test with a fresh sample.<\/p>\n\n
21. Tap \u201cOpen\/ Close Tray\u201d button to eject the chip tray.<\/p>\n\n
22. Lift the chip and discard (1% sodium hypochlorite).<\/p>\n\n
<\/p>\n"},"subContentId":"f8fb246c-3acb-4bdf-af72-f6701c283081","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""}],"slideBackgroundSelector":{}},{"elements":[{"x":2.178649237472767,"y":6.484881209503239,"width":92.5925925925926,"height":77.7537796976242,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
Amplification and Detection of MTB Rif<\/strong><\/span><\/p>\n\n
\n\t
DNA from the MTB positive elutes is tested for Rif-resistance using the MTB-Rif chip.<\/li>\n\t
Select the test profile \u201cMTB Rif\u201d.<\/li>\n\t
Select the sample type.<\/li>\n\t
Press \u201cStart Reaction\". For Truelab Uno Dx, Press the eject button to open the chip tray. For Truelab Duo\/Quattro, the chip tray opens automatically on tapping the \u201cStart Reaction\u201d.<\/li>\n\t
Take out \u201cTruenat MTB Rif Dx chip\u201d from the chip sleeve (check desiccant colour. If blue -chip can be used; discard chip if desiccant is pink\/ white which indicates moisture is absorbed).<\/li>\n\t
Follow steps 10-16 as described in Amplification and detection of MTB (above).<\/strong><\/span><\/li>\n\t
The test completes in 55 minutes, press \u2018\u2019RESULT\u2019\u2019 to view the result screen.<\/li>\n<\/ol>\n"},"subContentId":"e840fdf5-3daa-4a14-a44c-40ed27641651","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":2.178649237472767,"y":10.799136069114471,"width":34.85838779956427,"height":0.2159827213822894,"action":{"library":"H5P.Shape 1.0","params":{"line":{"borderWidth":"1","borderStyle":"dotted","borderColor":"#000"},"type":"horizontal-line","shape":{"fillColor":"#ffffff","borderColor":"#000000","borderWidth":"0","borderStyle":"solid","borderRadius":"0"}},"metadata":{"contentType":"Shapes","license":"U"},"subContentId":"3cd90916-7437-40aa-9031-893604d0356d"},"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false}],"slideBackgroundSelector":{}},{"elements":[{"x":3.2679738562091503,"y":10.75268817204301,"width":91.50326797385621,"height":49.676025917926566,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
8. Possible results:<\/p>\n\n
\n\t
MTB Rif resistance detected\/ MTB Rif resistance not detected\/ Error\/ Indeterminate.<\/li>\n<\/ul>\n\n
9. If result is Indeterminate\/ Error, repeat the amplification using the same extracted DNA and new \u201cTruenat MTB Rif Dx chip\u201d. If Indeterminate\/ Error again, repeat the test with a fresh sample.<\/p>\n\n
10. Tap \u201cOpen\/ Close Tray\u201d button to eject the chip tray.<\/p>\n\n
11. Lift the chip and discard (1% sodium hypochlorite).<\/p>\n\n
<\/p>\n"},"subContentId":"a9d149b8-906d-43b9-a815-0928d4007704","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""}],"slideBackgroundSelector":{}},{"elements":[{"x":2.178649237472767,"y":64.51612903225806,"width":27.233115468409586,"height":30.10752688172043,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
Figure: Truenat MTB Assay Results Interpretation: Algorithm; Source: Practical Guide to Implementation of Truenat Tests for the Detection of TB and Rifampicin Resistance<\/em><\/p>\n\n
<\/p>\n"},"subContentId":"0eb02420-f630-4c93-b392-7795b001bbd8","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":2.178649237472767,"y":2.159827213822894,"width":94.55337690631809,"height":8.639308855291576,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
The algorithm for Truenat MTB Assay (Figure) describes interpretation of test results obtained for Truenat MTB and Truenat MTB Rif Dx assays.<\/p>\n"},"subContentId":"022ad846-e443-467c-808d-845a3055b874","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":33.769063180827885,"y":8.602150537634408,"width":64.37908496732027,"height":91.39784946236558,"action":{"library":"H5P.Image 1.1","params":{"decorative":false,"contentName":"Image","expandImage":"Expand Image","minimizeImage":"Minimize Image","file":{"path":"images\/file-65decb422ac57.png","mime":"image\/png","copyright":{"license":"U"},"width":654,"height":541}},"subContentId":"5b180bee-7bbd-4e14-abf3-29ca4d7f1fdc","metadata":{"contentType":"Image","license":"U","title":"Untitled Image"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""}],"slideBackgroundSelector":{}},{"elements":[{"x":8.714596949891067,"y":4.319654427645788,"width":82.78867102396515,"height":93.9524838012959,"action":{"library":"H5P.Video 1.6","params":{"visuals":{"fit":true,"controls":true},"playback":{"autoplay":false,"loop":false},"l10n":{"name":"Video","loading":"Video player loading...","noPlayers":"Found no video players that supports the given video format.","noSources":"Video is missing sources.","aborted":"Media playback has been aborted.","networkFailure":"Network failure.","cannotDecode":"Unable to decode media.","formatNotSupported":"Video format not supported.","mediaEncrypted":"Media encrypted.","unknownError":"Unknown error.","invalidYtId":"Invalid YouTube ID.","unknownYtId":"Unable to find video with the given YouTube ID.","restrictedYt":"The owner of this video does not allow it to be embedded."},"sources":[{"path":"videos\/sources-6492611311b6b.mp4","mime":"video\/mp4","copyright":{"license":"U"}}]},"subContentId":"2a773259-a195-4d6e-8ea6-34bd86353903","metadata":{"contentType":"Video","license":"U","title":"Untitled Video"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""}],"slideBackgroundSelector":{}}],"keywordListEnabled":true,"globalBackgroundSelector":{},"keywordListAlwaysShow":false,"keywordListAutoHide":false,"keywordListOpacity":90},"override":{"activeSurface":false,"hideSummarySlide":false,"summarySlideSolutionButton":true,"summarySlideRetryButton":true,"enablePrintButton":true,"social":{"showFacebookShare":false,"facebookShare":{"url":"@currentpageurl","quote":"I scored @score out of @maxScore on a task at @currentpageurl."},"showTwitterShare":false,"twitterShare":{"statement":"I scored @score out of @maxScore on a task at @currentpageurl.","url":"@currentpageurl","hashtags":"h5p, course"},"showGoogleShare":false,"googleShareUrl":"@currentpageurl"}},"l10n":{"slide":"Slide","score":"Score","yourScore":"Your Score","maxScore":"Max Score","total":"Total","totalScore":"Total Score","showSolutions":"Show solutions","retry":"Retry","exportAnswers":"Export text","hideKeywords":"Hide sidebar navigation menu","showKeywords":"Show sidebar navigation menu","fullscreen":"Fullscreen","exitFullscreen":"Exit fullscreen","prevSlide":"Previous slide","nextSlide":"Next slide","currentSlide":"Current slide","lastSlide":"Last slide","solutionModeTitle":"Exit solution mode","solutionModeText":"Solution Mode","summaryMultipleTaskText":"Multiple tasks","scoreMessage":"You achieved:","shareFacebook":"Share on Facebook","shareTwitter":"Share on Twitter","shareGoogle":"Share on Google+","summary":"Summary","solutionsButtonTitle":"Show comments","printTitle":"Print","printIngress":"How would you like to print this presentation?","printAllSlides":"Print all slides","printCurrentSlide":"Print current slide","noTitle":"No title","accessibilitySlideNavigationExplanation":"Use left and right arrow to change slide in that direction whenever canvas is selected.","accessibilityCanvasLabel":"Presentation canvas. 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Truenat: Testing using Truenat

Target Audience

Unloading the Truenat Cartridge

Using Truenat autoanalyzer for MTB testing

Loading the elute onto the Truenat MTB Chip

Amplification and Detection of MTB and MTB-Rif