Node Status
Learning Objectives
Equip participants with the skills to manage culture isolates for tuberculosis (TB) diagnosis and drug susceptibility testing (DST).
- Understand and apply criteria for accepting MTB isolates for DST.
- Prepare MTB isolates following standard protocols for DST.
- Execute the preparation of isolates specifically for liquid culture DST.
- Implement proper storage techniques to maintain the viability of culture isolates.
- Identify and prepare necessary media and consumables for storing culture isolates.
- Prepare Selective Middlebrook-7H9 liquid medium and conduct quality control to ensure reliability.
- Perform accurate inoculation procedures for storing culture isolates.
- Conduct sterility checks to ensure culture isolates are contamination-free.
- Properly incubate and store culture isolates to maintain their integrity.
- Successfully revive frozen cultures while preserving their viability for future testing.
Sub Nodes
- Acceptance of MTB Isolates for DST
- Preparing the Isolate for DST
- Preparing the Isolate for Liquid Culture DST
- Incubation and Storage of Culture Isolates
- Preparation of Selective Middlebrook- 7H9 Liquid Medium
- Quality Control of Selective Middlebrook- 7H9 Liquid Medium
- Sterility Check of Culture Isolates
- Revival of the Frozen Cultures