Content Status
Type
Linked Node
Inoculum Preparation for Immunochromatographic Test SD Bioline Assay/Capillia/TBcID
Learning ObjectivesDescribe steps in inoculum preparation
Specimen preparation and subsequent steps must be performed in a BSC using full PPE.
From positive MGIT tubes:
-
Ideally, test AFB smear-positive MGIT tubes within 5 days of instrument positivity.
-
Vortex the tightly capped MGIT tube for 30 seconds to ensure the suspension is well-mixed.
From positive LJ slants:
-
Test 2 to 4-week-old growth.
-
Add 200μL of TBC ID extraction buffer or Capilia extraction buffer to a sterile cryovial.
-
Using a sterile 10 μL loop, scrape a loopful of several colonies and mix with buffer, avoiding any solid medium and/or contaminants present.
-
Vortex the cryovial for 30 seconds to create a uniform suspension.
Further steps are the same for both :
-
Place 100μL of the specimen, either MGIT culture or bacterial suspension from LJ slant, into the specimen well of the test device. Change pipette tips between specimens.
-
Start timer for 15 minutes.
-
Examine the reading area of the test device after 15 minutes and record the test results. Do not interpret the test after 60 minutes.
Resource
Assessment
Question 1 |
Answer 1 |
Answer 2 |
Answer 3 |
Answer 4 |
Correct Answer |
Correct Explanation |
Page id |
Part of Pre-Test |
Part of Post-Test |
The tightly capped MGIT tube should be vortexed for 30 seconds before performing the test. |
True |
False |
|
|
True |
The tightly capped MGIT tube must be vortexed for 30 seconds to ensure the suspension is well-mixed.
|
|
Yes |
Yes |
Question 2 |
|
|
|
|
|
|
|
|
|
The reading of the immunochromatographic test should not be taken after 60 min. |
True |
False |
|
|
True |
Do not interpret the test after 60 minutes.
|
|
Yes |
Yes |
Content Creator
Reviewer
Target Audience
- Log in to post comments