Content Status
Type
Linked Node
Procedure of Quality Control for DST
Learning ObjectivesQC procedure and corrective action, validation of new methods/procedures/consumables
Quality controls procedures are essential to generate quality DST results. Quality procedures are essential when using QC strains and reagents and preparing and using drugs for DST. These include:
QC strains
1. Use well-characterized pan-susceptible M. TB strain/ H37Rv/ M. tuberculosis ATCC 27294
2. Colonies from solid media less than 14 days old
3. MGIT 960 tube 1–5 days after flagged positively by instrument
4. Additional organisms may be tested to supplement BD QC recommendations
5. Test with a known pan-susceptible strain of M. tuberculosis
Drug and Inoculum Preparation
1. Ensure proper reconstitution of lyophilized drugs by following the given guidelines:
- Rehydrate drugs with sterile distilled water
- Thaw aliquots of prepared drugs from freezer
- Check the expiry date before use
2. Avoid wrong drug concentrations.
3. Avoid improper drug preparation.
4. Proper dilution of inoculum for drug and growth control tube is critical.
5. Suspension must be well mixed and homogeneous without clumps.
6. Prepare QC inoculum (H37Rv) suspension in the same way as patient isolate suspension.
7. Use positive and negative controls.
8. Follow standard operating procedures to perform SIRE/ Second-line and PZA DSTs.
Resource
GLI Practical Guide to TB Laboratory Strengthening
Assessment
Question
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Answer 1
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Answer 2
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Answer 3
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Answer 4
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Correct answer
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Correct explanation
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Page id
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Part of Pre-test
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Part of Post-test
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Which of the following procedures are included in the quality control of MGIT DST?
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Prepare QC inoculum (H37Rv) suspension in the same way as patient isolate suspension.
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Use well-characterized pan-susceptible M. TB strain/ H37Rv/ M. tuberculosis ATCC 27294 .
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Ensure proper reconstitution of lyophilized drugs.
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All |
4
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Quality procedures are essential when using QC strains and reagents and preparing and using drugs for DST.
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YES
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YES
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