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The CBNAAT Cartridge
Learning ObjectivesDiscuss the parts of the CBNAAT/ GeneXpert Cartridge and its functions.
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The disposable, single-use CBNAAT cartridge is a closed system unit to hold the samples and reagents. Inside of a CBNAAT Cartridge (CBNAAT operator manual). Each cartridge consists of the following components:
- Processing chambers: Hold samples, reagents, processed samples, and waste solutions. One chamber is designated as an air chamber to equilibrate pressures within the cartridge.
- Valve body: Rotates and allows fluid to move to different cartridge chambers and to the reaction tube. Within the valve body, the specimen is isolated, PCR inhibitors are removed, and the sample is mixed with PCR reagents and moved into the integrated reaction tube.
- Reaction tube: Enables rapid thermal cycling and optical excitation and detection of the tube contents. The reaction tube is automatically inserted into the I-CORE module (hardware for PCR amplification and fluorescence detection) when the cartridge is loaded into the instrument.
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